Molecular Biology/Plasmid Constructs Co-Op

Legend Biotech is a global biotechnology company dedicated to treating, and one day curing, life-threatening diseases. Headquartered in Somerset, New Jersey, we are developing advanced cell therapies across a diverse array of technology platforms, including autologous and allogenic chimeric antigen receptor T-cell, T-cell receptor (TCR-T), and natural killer (NK) cell-based immunotherapy. From our three R&D sites around the world, we apply these innovative technologies to pursue the discovery of safe, efficacious and cutting-edge therapeutics for patients worldwide.

Legend Biotech entered into a global collaboration agreement with Janssen, one of the pharmaceutical companies of Johnson & Johnson, to jointly develop and commercialize ciltacabtagene autolecuel (cilta-cel). Our strategic partnership is designed to combine the strengths and expertise of both companies to advance the promise of an immunotherapy in the treatment of multiple myeloma.

Legend Biotech is seeking a Molecular Biology/Plasmid Constructs Co-Op as part of the R&D team based in Philadelphia, PA.

Role Overview

The Molecular Biology/Plasmid Constructs Co-Op will contribute to the design and functional validation of synthetic and natural promoters for T-cell gene expression, leveraging bioinformatics tools and molecular biology techniques. This role combines computational analysis with hands-on lab work to advance gene regulation strategies in cutting-edge gene therapy research.

Co-Op Duration: 6-month (Summer to Fall) with possible extension.

Key Responsibilities

• Assist in designing synthetic and natural promoters for T-cell specific gene expression using bioinformatics tools and literature mining.
• Perform cloning of promoter sequences into lentiviral vectors using standard molecular biology techniques (PCR, restriction digestion, ligation, Gibson assembly).
• Maintain mammalian cell lines and primary T cells; support transfection and transduction experiments.
• Conduct reporter assays (e.g. luciferase, GFP) to evaluate promoter strength and specificity in T cells or cell lines.
• Analyze experimental data using software such as GraphPad Prism, Excel, and basic statistical methods.
• Maintain accurate lab notebooks and present findings in team meetings.

Requirements

• Currently enrolled in Graduate programs (Master's) in bioinformatics, data science, computer science, computational biology, or related field
• Basic understanding of molecular biology and cell culture principles
• Strong interest in promoter engineering and gene regulation with an understanding of transcriptional control mechanisms
• Experience with bioinformatics tools or programming languages (Python, R) is a plus
• Familiarity with laboratory techniques such as pipetting, buffer preparation, and aseptic handling.
• Strong attention to detail, organizational skills, and ability to work independently and in a team environment
• Excellent communication and documentation skills.

What we offer:

• Exposure to cutting-edge gene therapy and synthetic biology.
• Practical experience in promoter engineering and functional validation.
• Mentorship from experienced scientists in a collaborative team setting.

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Please note: These benefits are offered exclusively to permanent full-time employees. Contractors are not eligible for benefits through Legend Biotech.

EEO Statement

It is the policy of Legend Biotech to provide equal employment opportunities without regard to actual or perceived race, color, creed, religion, national origin, ancestry, citizenship status, age, sex or gender (including pregnancy, childbirth, related medical conditions and lactation), gender identity or gender expression (including transgender status), sexual orientation, marital status, military service and veteran status, disability, genetic information, or any other protected characteristic under applicable federal, state or local laws or ordinances.

Employment is at-will and may be terminated at any time with or without cause or notice by the employee or the company.

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