Research Technician, Biochemistry, Meydan Lab

ABOUT THE WORK UNIT

The Meydan Lab is interested in the mechanisms of protein synthesis, and the role of translation regulation and ribosomes in human health. We use various biochemistry techniques, sequencing of ribosome protected mRNA fragments, (a.k.a ribosome profiling (Ribo-seq), disome profiling (Disome-seq), and mitoribosome profiling), and RNA-seq in model systems of Saccharomyces cerevisiae (budding yeast), immortalized human cells, and neurons differentiated from induced pluripotent stem cells (iPSCs) to address our questions.

KEY FUNCTIONS AND EXPECTED PERFORMANCE

Assists in Research and Development:

• Researches and collects data using appropriate lab experiments, procedures, and techniques
• Preparation of buffers and reagents
• Production and maintenance of plasmids, yeast strains and mammalian cell lines
• Collects, analyzes, and interprets data with assistance of other members of the laboratory
• Writes and updates laboratory protocols
• Reports on status of research activities in oral and written formats
• Assembles and compile research data for presentation and/or reports

Maintenance:

• Conducts general lab maintenance
• Assists in set up, operation, and maintenance of laboratory equipment including, but not limited to - biosafety cabinets, incubators, centrifuges, etc.
• Proper storage and maintenance of DNA and virus stocks

Performs administrative duties:

• Monitors inventory of supplies and assists with ordering
• Participate in weekly and bimonthly meetings to discuss project progress
• Other tasks as directed by supervisor

SUPERVISORY RELATIONSHIPS

• This position does not have supervisory responsibility.
• The position reports administratively and functionally to the PI.

EDUCATION AND CERTIFICATIONS

• A Bachelor's degree in biochemistry, biological sciences, chemistry, or related field from an accredited institution of higher education is necessary.

EXPERIENCE AND SKILLS

• Knowledge and experience with mammalian cell culture and protein expression is preferred.
• Knowledge of virus expression and purification is preferred.
• Knowledge of electron microscopy is preferred.
• Knowledge of fluorescence microscopy is preferred.
• Experience with organization, inventory, and record keeping is preferred.
• Excellent organizational and communications skills (both verbal and written) are preferred.